Caulobacter and Asticcacaulis stalk bands as indicators of stalk age

Determinationof Caulobacter Crescentus Stalk Function throughHyphenatedProteomics Techniques

Physiological role of stalk lengthening in Caulobacter crescentus

The Gram-negative bacterium Caulobacter crescentus forms a thin polar stalk, which mediates its attachment to solid surfaces. Whereas stalks remain short (1 µm) in nutrient-rich conditions, they lengthen dramatically (up to 30 µm) upon phosphate starvation. A long-standing hypothesis is that the Caulobacter stalk functions as a nutrient scavenging "antenna" that facilitates phosphate uptake and...

Phospholipid biosynthesis is required for stalk elongation in Caulobacter crescentus.

Membrane phospholipid synthesis was inhibited in Caulobacter crescentus by growth of a glycerol auxotroph in the absence of glycerol or by treatment with the antibiotic cerulenin. It was observed that the final step in the swarmer cell-to-stalked cell transition, stalk elongation, was inhibited under these conditions. Since an early effect of inhibiting phospholipid synthesis in C. crescentus i...

Crinoid stalk flexibility: theoretical predictions and fossil stalk postures

Flexibilities in Lower Mississippian crinoid stalks were inferred from preserved postures and shown not to conform with predictions of a cantilever beam model. Flexibilities were not correlated with hard-part characters such as stalk diameter, stalk length, or columnal height. The lack of correspondence between the predictions of the cantilever model and observed flexibilities implies that the ...

Caulobacter crescentus requires RodA and MreB for stalk synthesis and prevention of ectopic pole formation.

Caulobacter crescentus cells treated with amdinocillin, an antibiotic which specifically inhibits the cell elongation transpeptidase penicillin binding protein 2 in Escherichia coli, exhibit defects in stalk elongation and morphology, indicating that stalk synthesis may be a specialized form of cell elongation. In order to investigate this possibility further, we examined the roles of two other...